Evidence-based refinements for pigs.
Housing & Handling
Key Natural Behaviors
- Social with tightly bonded social groupings and dominance hierarchies
- Foraging behaviors such as rooting, grazing, and exploration
- Diurnal with long periods of rest/sleep & long periods of activity
- Highly intelligent
- Social facilitation (synchronization of activities such as eating, sleeping, manipulating materials)
Recommendations
- Stable social groupings
- If adult pigs must be mixed use pens designed with extra space, getaway areas/barriers, & management techniques
- If pigs must be singly housed, allow physical contact through wire mesh or similar
- Floor pens with rough surface flooring and straw/shaving/sawdust bedding substrate to prevent slipping and promote rooting
- If facility limitations prevent the use of such bedding, plastic or rubber mats can be used
- Pen large enough to accommodate a defecation area separate from the feeding area
- Behavioral management
- Positive reinforcement, socialization, & habituation training for routine handling/procedures
- Staff should receive proper training before implementing these procedures.
- Robust enrichment schedule including multiple rooting, chewing, and play opportunities.
- Key enrichment characteristics: edible, chewable, investigable, and manipulable (e.g., snout-operated pellet dispenser)
- If possible, enough enrichment materials should be provided for all pigs to access them simultaneously to avoid aggression.
- If possible, outdoor access with an activity area such as access to a pool and rooting area filled with bark and wood chips.
Procedural Refinements
1. Swine Restraint
- Positive Reinforcement Training
- Use of Swine Board
- Peanut Butter on Wall
- Patience is everything!
2. Swine Anaesthesia
- Gatoraid and Prang Popsicles for NPO
- 3D Refinements
- Ear Wedges
- Modify recovery surroundings:
- Soft padding during recovery
- Low light
- Low voice
- Dedicated post-op nursing care
- John Hopkins Chair
3. Swine Humane Euthanasia
- Pre-Medication (Sedative): Injectable and Oral options
- Euthanasia Options
- Adequate Restraint: It’s all in the hold
4. Swine Blood Collection
- Consistent body weight
- Place weigh scale at door entryway
- Consistent anesthesia
- Positive Reinforcement Training
Related Refinements
Behavioural Management Program
- Dedicated behavior manager to ensure consistency
- Dedicated environmental enrichment opportunities
- Rotational
- Human-Patient Interaction
- Understanding time to learn individual behavior
Further Reading
General
- The Pig. In Animal-Centric Care & Management: Enhancing refinement in biomedical research.
- Comfortable Quarters for Laboratory Animals
- Animal Welfare Institute’s Refinement Database: Pig
- Smith & Swindle. 2006. Preparation of Swine for the Laboratory, ILAR Journal.
- Ellegaard L et al. 2010. Welfare of the minipig with special reference to use in regulatory toxicology studies. J Pharmacol Toxicol Methods.
- Minipig Resource List
Social Housing
Enrichment
- van de Weerd & Day. 2009. A review of environmental enrichment for pigs housed in intensive housing systems
- Mkwanazi et al. 2019. Effects of environmental enrichment on behaviour, physiology and performance of pigs – A review
Handling & Positive Reinforcement Training
- Malley et al. 2022. Refining restraint techniques for research pigs through habituation.
- Garza & Cannon. 2020 Piggy see, piggy do: A swine training program enhances animal welfare and research.
- Sørensen. 2010. “Never wrestle with a pig.”
- The UFAW Handbook on the Care and Management of Laboratory and Other Research Animals. Germany, Wiley, 2010. Chapter 16 Positive Reinforcement Training for Laboratory Animals. Gail Laule.
Blood Sampling
- NC3Rs Pig Blood Sampling
- Cranial Vena Cava
- Ear Vein
- Saphenous Cannulation
- External Juglar Vein
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