Evidence-based refinements for mice
A mouse peeks out of a well-formed nest.
Key Natural Behaviors
- Social structure
- Nesting
- Burrowing
- Climbing
- Foraging
- Nocturnal/crepuscular
- Avoid open spaces
Recommendations
- Stable group housing
- Water/food at one end
- Nesting material – a minimum of 8 grams for 3-5 mice. (Transfer clean & dry nesting material during cage change.)
- Aspen bedding is generally preferable compared to corn cob
- Tubes & climbing structures
- Avoid cages being in the direct path of ultrasound when possible (dripping taps, cart wheels, computers)
- If using individually ventilated cages, monitor for anxiety/discomfort based on high air change rate
- Running wheels
- Gnawing sticks
- Avoid bright light (mice prefer <65 lux) especially covering the top row of cages on a rack
- Grid space, mouse lofts, or mezzanines
- Tunnel handling or cupping: see our Rodent Handling page for tips
